次世代シーケンス

マクロジェンは、様々なシーケンシングプラットフォームに特化した解析ソフトウェアを利用して、 Standard Data Report と解析結果を提供します。 専門の解析サービスチームがお客様の多様なニーズに対応するカスタム解析も提供できます。また、遺伝変異の確認から比較解析に至るまで、付加的な解析を行っています。

NGS platform and Application available at Macrogen

  • De novo Assembly / Gene Annotation
  • Variant Calling (SNP, In/Del) and Annotation
  • Structural Variation (CNV and SV) and Annotation
  • Expression Profiles
  • Differentially Expressed Genes (DEGs) / miRNA
    Enrichment Profilies / Gene Annotation
  • Gene Ontology / Gene Set Analysis / Pathway Analysis
  • Operational Taxonomic Unit (OTU) Diversity

Bio Informatics Services

Sequence
Methodology		 Sequence
Only		 Standard Analysis Special Analysis
De novo
Sequencing		 FASTQ De novo assembly
  • 1. Variant Calling (SNP, In/Del)
  • 2. Gene Prediction
  • 3. Gene Annotation
Whole
Genome
Resequencing		 FASTQ
  • 1.Alignment and summary of data production
  • 2.Variant Calling (SNP, In/Del) and Annotation
  • 1.Comparative Analysis
  • 2.Disease Analysis
  • 3.Structural Variation (CNV and SV) and    Annotation
Exome /Target
Sequencing		 FASTQ
  • 1.Alignment and summary of data production
  • 2.Variant Calling (SNP, In/Del) and Annotation
  • 1.Comparative Analysis
  • 2.Disease Analysis
Transcriptome
Resequencing		 FASTQ
  • 1.Expression Profiles
  • 2.Differentially Expressed Genes (DEGs)
  • 3.Fusion Gene
  • 1.Gene Set Analysis
  • 2.Variant Calling and Annotation
  • 3.Comparative Analysis
Transcriptome
De Novo
Sequencing		 FASTQ
  • 1.De novo Assembly
  • 2.Alignment and Statistics
  • 3.Expression Profiling
  • 1.Gene Annotation
  • 2.Gene Set Analysis
  • 3.Variant Calling (SNP, In/Del) and    Annotation
smRNA
Sequencing		 FASTQ
  • 1.Expression Profiles
  • 2.Novel smRNA
  • 3.Differentially Expressed miRNA
  • 1.Target Prediction of Known and Novel
       miRNA
  • 2.Gene Set Analysis
  • 3.Comparative Analysis
Metagenome
16s rDNA
Sequencing		 FASTQ
  • 1.OTU Counting
  • 2.OTU Diversity
  • Comparative Analysis
Metagenome
Shotgun
Sequencing		 FASTQ
  • 1.De novo assembly
  • 2.Blastx
  • 1.Gene Prediction
  • 2.OTU counting (16S rDNA or other
       housekeeping genes)
  • 3.Function assignment to each gene   candidate
  • 4.Constructing metabolic pathways
  • 5.Statistical analysis of two or more
       meta-genomic samples
ChIP
Sequencing		 FASTQ
  • 1.Enrichment Peak Profiles and Gene Annotation
  • 2.Differentially Enriched Peaks and Gene Annotation
  • 3.Multiple Alignment and Consensus Motif
  • 1.Gene Set Analysis
  • 2.Comparative Analysis
MBD Sequencing FASTQ
  • 1.Global Methylation Profiles
  • 2.Specific Methylation Profiles
    • - Protein-Coding Genes
    • - CG Islands
    • - Several Repeated Sequences
  • 3. Differentially Methylation Regions (DMR)
  • 1.DMR-Associated Genes
  • 2.Gene Set Analysis
  • 3.Comparative Analysis